Tikal: 4D Image Processing Software
Mrs Corinna Sprengart
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4-D image processing platform to analyse data derived from laser scanning and wide field microscopes.
Type of Transfer (explanation):
Commercial Potential: yes
Quantity per Transfer: 1 licenses
Existing patent or patent pending: no
Restricted User License (explanation): no
Other Controling Interests: no
Offer Expires: 0 seconds
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Developers / Researchers: Bacher Christian (DKFZ), Eils Roland (DKFZ)
Where Eils Roland is supervisor/ Snr Researcher.
TIKAL software visualizes global movements and local deformations of cells as well as facilitating the 2-D and/or 3-D tracking of particles. It allows the automatic 3-D analysis of particle movement in parallel with the acquisition of chromatin density data.
- To study the dynamics of two different types of nuclear particles, namely nuclear bodies made from GFP-NLS-vimentin and microinjected 0.1 μm – wide polystyrene beads, by live cell time-lapse microscopy combined with single particle tracking and mobility analysis. see: 4-D single particle tracking of synthetic and proteinaceous microspheres reveals preferential movement of nuclear particles along chromatin – poor tracks.
Christian P Bacher, Michaela Reichenzeller, Chaitanya Athale, Harald Herrmann, and Roland Eils BMC Cell Biol. 2004; 5: 45.
- PML NBs were shown to exhibit directed movement when progressing from prophase to prometaphase by using three-dimensional time-lapse live cell imaging and four-dimensional particle tracking. see: Live Cell Dynamics of Promyelocytic Leukemia Nuclear Bodies upon Entry into and Exit from Mitosis. Yi-Chun M. Chen, Constantin Kappel, Joel Beaudouin, Roland Eils, and David L. Spector Mol Biol Cell. 2008 July; 19(7): 3147–3162.
- Single-particle tracking methods were used to examine the mobility of two subnuclear organelles (Promyelocytic leukemia (PML) and Cajal bodies) in comparison to aggregates of murine Mx1 (biochemically inactive). see: Nuclear body movement is determined by chromatin accessibility and dynamics. Sabine M. Görisch, Malte Wachsmuth, Carina Ittrich, Christian P. Bacher, Karsten Rippe, and Peter Lichter Proc Natl Acad Sci U S A. 2004 September 7; 101(36): 13221–13226.
- Quantitative spatially resolved scaling analysis (SRSA) was possible by segmenting the nucleus from confocal laser scanning microscopy stacks (using Tikal) in the anaylsis of the decondensation of interphase chromatin in Trichostatin A-induced histone acetylation. see: Trichostatin A-induced histone acetylation causes decondensation of interphase chromatin. Journal of Cell Science 117, 4277-4287 Published by The Company of Biologists 2004 doi:10.1242/jcs.01293
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